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m isn't confined by the association price constant for the formation with the NagA-substrate complicated. These effects (along with the improved fee of response of compound six relative to compound 1) are only according to the conclusion that NagA is restricted by possibly the formation or breakdown in the tetrahedral intermediate.

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-acetyl derivative. This result is in line with the formation or collapse from the tetrahedral intermediate as the rate limiting move in the catalytic system of NagA.

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Within the crystal structure of the D273N mutant of NagA, the side chain of His-143 is three.0 Å faraway from the phosphonate oxygen from the mimic in the tetrahedral intermediate (21). This end result suggests that this team could aid the activation with the carbonyl team on the substrate along with an interaction With all the metallic ion certain in the active web site.

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